rabbit anti mt1  (Huabio Inc)

Journal: Animals : an open access journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

doi: 10.3390/ani15010083

Figure Lengend Snippet: Figure 3. Distribution of MT1 melatonin receptors in camel spermatozoa, evaluated by the indirect immunofluorescence method. Immunostaining in the head (H), post-acrosome (PA), neck (N), tail (T) and cytoplasmic droplet (CD) was evidenced. Magnification 1000×. MT1 receptors (A,D,G,J), Hoechst staining (B,E,H,K), and bright field (C,F,I,L) are shown.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, Animals 2025, 15, 83 5 of 17 RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 ◦C in a wet chamber.

Techniques: Immunofluorescence, Immunostaining, Staining

Journal: Animals : an open access journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

doi: 10.3390/ani15010083

Figure Lengend Snippet: Figure 5. Indirect immunofluorescence controls. Samples were incubated with only the MT1 (A) or MT2 (C) primary or secondary antibody (E) and (G) for Alexa Fluor 594 anti-mouse antibody and Alexa Fluor 488 anti-rabbit antibody, respectively). Magnification 1000×. Fluorescence after 30 s exposition (A,C,E,G) and bright field (B,D,F,H) are shown.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, Animals 2025, 15, 83 5 of 17 RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 ◦C in a wet chamber.

Techniques: Immunofluorescence, Incubation, Fluorescence

Journal: Animals : an open access journal from MDPI

Article Title: Melatonin in Male Dromedary Camel ( Camelus dromedarius ) Seminal Plasma and Its Specific MT1 and MT2 Receptors on Sperm Membranes.

doi: 10.3390/ani15010083

Figure Lengend Snippet: Figure 6. Comparison among age classes of males at different melatonin receptors localizations (MT1 (A) and MT2 (B)) in acrosome (A), post-acrosome (PA), head (H), neck (N), tail (T), cytoplasmic droplet (CD) and apical edge (AE). Values are shown as percentage of localization of n = 439 spermatozoa. * indicates p < 0.05.

Article Snippet: Following three additional PBS washes, the spermatozoa were incubated with the primary antibody for melatonin receptor MT1 (MTNR1A mouse polyclonal antibody; Abnova, Taipei, Taiwan; Cat# H00004543-A01, Animals 2025, 15, 83 5 of 17 RRID: AB_462681) diluted 1:10 in PBS with 1% BSA, or the primary antibody for melatonin receptor MT2 (MTNR1B Rabbit Polyclonal Antibody, Acris Antibodies GmbH, Herford, Germany; Cat# AP01322PU-N, RRID: AB_1619198) diluted 1:20 in PBS with 1% BSA overnight at 4 ◦C in a wet chamber.

Techniques: Comparison